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验证数据展示

  • IF Staining of HepG2 using CL488-67303

    IF Staining of HepG2 using CL488-67303

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ADH4 antibody (CL488-67303, Clone: 3D12E9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for IF/ICC
Cat No. CL488-67303

产品说明书

CloneNo. 3D12E9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

ADH 2, ADH4, Alcohol dehydrogenase 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67303-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67303 targets ADH4 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 ADH4 fusion protein Ag9884 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alcohol dehydrogenase 4 (class II), pi polypeptide
别名 ADH 2, ADH4, Alcohol dehydrogenase 4
计算分子量 380 aa, 40 kDa
观测分子量 40-43 kDa
GenBank蛋白编号BC022319
基因名称 ADH4
Gene ID (NCBI) 127
RRIDAB_2934532
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08319
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ADH4 antibody CL488-67303Download protocol
Standard Protocols
Click here to view our Standard Protocols