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CoraLite® Plus 488-conjugated ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D12E9

Cat no : CL488-67303

Print datasheet

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

验证数据展示

  • IF Staining of HepG2 using CL488-67303

    IF Staining of HepG2 using CL488-67303

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ADH4 antibody (CL488-67303, Clone: 3D12E9 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67303-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67303 targets ADH4 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen ADH4 fusion protein Ag9884 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Synonyms ADH 2, ADH4, Alcohol dehydrogenase 4
Calculated Molecular Weight 380 aa, 40 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08319
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ADH4 antibody CL488-67303Download protocol
Standard Protocols
Click here to view our Standard Protocols