验证数据展示
经过测试的应用
Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | 3T3-L1 cells |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83961-3-RR targets Adiponectin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Adiponectin fusion protein Ag16304 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | adiponectin, C1Q and collagen domain containing |
Synonyms | ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein |
Calculated Molecular Weight | 244 aa, 26 kDa |
GenBank Accession Number | BC096308 |
Gene Symbol | Adiponectin |
Gene ID (NCBI) | 9370 |
RRID | AB_3671541 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15848 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.
实验方案
Product Specific Protocols | |
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IHC protocol for Adiponectin antibody 83961-3-RR | Download protocol |
IF protocol for Adiponectin antibody 83961-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |