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验证数据展示

  • IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Adiponectin antibody included
  • IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    IHC staining of mouse brown adipose using 83961-3-RR (same clone as 83961-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 83961-3-RR (ADIPOQ antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Adiponectin antibody included
  • IF Staining of 3T3-L1 using 83961-3-RR (same clone as 83961-3-PBS)

    IF Staining of 3T3-L1 using 83961-3-RR (same clone as 83961-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using ADIPOQ antibody (83961-3-RR, Clone: 241082E1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83961-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00919-2

    Cytometric bead array standard curve of MP00919-2

    Cytometric bead array standard curve of MP00919-2, Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83961-1-PBS. Detection antibody: 83961-3-PBS. Standard: Ag16304. Range: 2.5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83961-3-RR

    Affinity and Kinetic Characterization of 83961-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83961-3-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.

Adiponectin Recombinant antibody, PBS Only (Detector)

Adiponectin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83961-3-PBS

产品说明书

CloneNo. 241082E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Adiponectin Ready-To-Use IHC Kit. Adiponectin primary antibody included.

产品信息

83961-3-PBS targets Adiponectin as part of a matched antibody pair:

MP00919-2: 83961-1-PBS capture and 83961-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Adiponectin fusion protein Ag16304 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adiponectin, C1Q and collagen domain containing
别名 ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein
计算分子量 244 aa, 26 kDa
GenBank蛋白编号BC096308
基因名称 Adiponectin
Gene ID (NCBI) 9370
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15848
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.