ADRA1B Polyclonal antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22419-1-PBS

Synonyms

FC experiment of PC-3 using 22419-1-AP (same clone as 22419-1-PBS)
1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.
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1x10^6 PC-3 cells were intracellularly stained with 0.8 ug ADRA1B Polyclonal antibody (22419-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.
WB analysis using 22419-1-AP (same clone as 22419-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22419-1-AP (ADRA1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22419-1-PBS in a different storage buffer formulation.

Size

22419-1-PBS targets ADRA1B in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, Indirect ELISA Application Description
Tested Reactivity	human, mouse, rat
Immunogen	ADRA1B fusion protein Ag18138 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	adrenergic, alpha-1B-, receptor
Synonyms
Calculated Molecular Weight	520 aa, 57 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC136569
Gene Symbol	ADRA1B
Gene ID (NCBI)	147
RRID	AB_2879103
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	P35368
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C