ADRBK1 Monoclonal antibody
ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (2)
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
2F7A5
验证数据展示
经过测试的应用
| Positive WB detected in | THP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human lymphoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67067-1-Ig targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | ADRBK1 fusion protein Ag5071 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | adrenergic, beta, receptor kinase 1 |
| Synonyms | ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2 |
| Calculated Molecular Weight | 689 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC037963 |
| Gene Symbol | ADRBK1 |
| Gene ID (NCBI) | 156 |
| RRID | AB_2882377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25098 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IHC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| IF protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| FC protocol for ADRBK1 antibody 67067-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
