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ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

2F7A5

Cat no : 67067-1-Ig

Print datasheet

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

验证数据展示

  • WB analysis using 67067-1-Ig

    WB analysis using 67067-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 67067-1-Ig

    IF Staining of Jurkat using 67067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 67067-1-Ig

    FC experiment of HeLa using 67067-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADRBK1 (67067-1-Ig, Clone:2F7A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67067-1-Ig targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF, CoIP
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen ADRBK1 fusion protein Ag5071 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name adrenergic, beta, receptor kinase 1
Synonyms ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2
Calculated Molecular Weight 689 aa, 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC037963
Gene Symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_2882377
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IHC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IF protocol for ADRBK1 antibody 67067-1-IgDownload protocol
FC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Phytother Res

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB

Authors - Mengya Jiang
View Article