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验证数据展示

  • WB analysis using 67067-1-Ig

    WB analysis using 67067-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 67067-1-Ig

    IHC staining of human lymphoma using 67067-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 67067-1-Ig

    IF Staining of Jurkat using 67067-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 67067-1-Ig

    FC experiment of HeLa using 67067-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADRBK1 (67067-1-Ig, Clone:2F7A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67067-1-Ig

产品说明书

CloneNo. 2F7A5
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human and More (2)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

别名

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, U-937 cells, HL-60 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67067-1-Ig targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 Human
文献引用反应性human, mouse
免疫原 ADRBK1 fusion protein Ag5071 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 adrenergic, beta, receptor kinase 1
别名 ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2
计算分子量 689 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC037963
基因名称 ADRBK1
Gene ID (NCBI) 156
RRIDAB_2882377
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25098
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IHC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
IF protocol for ADRBK1 antibody 67067-1-IgDownload protocol
FC protocol for ADRBK1 antibody 67067-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF,CoIP

Phytother Res

Paeoniflorin-6'-O-benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of GRK2 and β-arrestin 2 on ERK1/2-NF-κB

Authors - Mengya Jiang
View Article