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验证数据展示

  • FC experiment of HeLa using CL594-67067

    FC experiment of HeLa using CL594-67067

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ADRBK1 (CL594-67067, Clone:2F7A5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Jurkat using CL594-67067

    IF Staining of Jurkat using CL594-67067

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 ADRBK1 antibody (CL594-67067, Clone: 2F7A5 ) at dilution of 1:200.

CoraLite®594-conjugated ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67067

产品说明书

CloneNo. 2F7A5

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67067-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67067 targets ADRBK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 ADRBK1 fusion protein Ag5071 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 adrenergic, beta, receptor kinase 1
别名 ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2
计算分子量 689 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC037963
基因名称 ADRBK1
Gene ID (NCBI) 156
RRIDAB_3084744
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25098
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
FC protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
Standard Protocols
Click here to view our Standard Protocols