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验证数据展示

  • FC experiment of HeLa using CL594-67067

    FC experiment of HeLa using CL594-67067

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ADRBK1 (CL594-67067, Clone:2F7A5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Jurkat using CL594-67067

    IF Staining of Jurkat using CL594-67067

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®594 ADRBK1 antibody (CL594-67067, Clone: 2F7A5 ) at dilution of 1:200.

CoraLite®594-conjugated ADRBK1 Monoclonal antibody

ADRBK1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67067

Print datasheet

CloneNo. 2F7A5

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67067-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67067 targets ADRBK1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen ADRBK1 fusion protein Ag5071 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name adrenergic, beta, receptor kinase 1
Synonyms ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2
Calculated Molecular Weight 689 aa, 80 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC037963
Gene Symbol ADRBK1
Gene ID (NCBI) 156
RRIDAB_3084744
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25098
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
FC protocol for CL594 ADRBK1 antibody CL594-67067Download protocol
Standard Protocols
Click here to view our Standard Protocols