Perilipin-2 Monoclonal antibody

Perilipin-2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60340-1-Ig

产品说明书

CloneNo. 1G5D4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

ADRP/Perilipin-2, ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman adipose tissue
Positive IHC detected inhuman renal cell carcinoma tissue, mouse brown adipose tissue, rat brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60340-1-Ig targets Perilipin-2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Perilipin-2 fusion protein Ag7643 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 adipose differentiation-related protein
别名 ADRP/Perilipin-2, ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2
计算分子量 48 kDa
观测分子量 47-52 kDa
GenBank蛋白编号BC005127
基因名称 Perilipin-2
Gene ID (NCBI) 123
RRIDAB_2881449
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99541
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

实验方案

Product Specific Protocols
WB protocol for Perilipin-2 antibody 60340-1-IgDownload protocol
IHC protocol for Perilipin-2 antibody 60340-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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