Perilipin-2 Recombinant antibody
Perilipin-2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
230274F4
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, HepG2 cells, K-562 cells |
Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | oleic acid treated HeLa cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80362-2-RR targets Perilipin-2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | Perilipin-2 fusion protein Ag7539 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | adipose differentiation-related protein |
Synonyms | ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC005127 |
Gene Symbol | Perilipin-2 |
Gene ID (NCBI) | 123 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q99541 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
IHC protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
IF protocol for Perilipin-2 antibody 80362-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |