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验证数据展示

  • IF Staining of HeLa using CL488-80362-2

    IF Staining of HeLa using CL488-80362-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP antibody (CL488-80362-2, Clone: 230274F4 ) at dilution of 1:200, CL594-phalloidin (red).

  • FC experiment of HepG2 using CL488-80362-2

    FC experiment of HepG2 using CL488-80362-2

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 ADRP Recombinant Antibody (CL488-80362-2, Clone:230274F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Perilipin-2 Recombinant antibody

Perilipin-2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-80362-2

产品说明书

CloneNo. 230274F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inoleic acid treated HeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80362-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80362-2 targets Perilipin-2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 Perilipin-2 fusion protein Ag7539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adipose differentiation-related protein
别名 ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4
计算分子量 48 kDa
观测分子量48 kDa
GenBank蛋白编号BC005127
基因名称 Perilipin-2
Gene ID (NCBI) 123
RRIDAB_3673032
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99541
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Perilipin-2 antibody CL488-80362-2Download protocol
Standard Protocols
Click here to view our Standard Protocols