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AEBP2 Monoclonal antibody

AEBP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7H2B11

Cat no : 66141-1-Ig

Print datasheet

Synonyms

AE binding protein 2, AEBP2, Zinc finger protein AEBP2

验证数据展示

  • WB analysis of HeLa using 66141-1-Ig

    WB analysis of HeLa using 66141-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66141-1-Ig (AEBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66141-1-Ig is AEBP2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, pig
Immunogen AEBP2 fusion protein Ag17905 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name AE binding protein 2
Synonyms AE binding protein 2, AEBP2, Zinc finger protein AEBP2
Calculated Molecular Weight 33 kDa, 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC015624
Gene Symbol AEBP2
Gene ID (NCBI) 121536
RRIDAB_2881538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6ZN18
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AEBP2 belongs to the AEBP2/jing C2H2-type zinc-finger family. It is a DNA-binding transcriptional repressor. AEBP2 is a zinc finger protein that has been shown to interact with the mammalian Polycomb Repression Complex 2 (PRC2). It may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. AEBP2 is an evolutionarily well-conserved gene that is found in the animals ranging from flying insects to mammals. (PMID:19293275) The antibody recognizes adult-specific larger form (52 kDa) and the embryo-specific smaller form (31 kDa).

实验方案

Product Specific Protocols
WB protocol for AEBP2 antibody 66141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols