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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83878-5-RR (same clone as 83878-5-PBS)

    WB analysis using 83878-5-RR (same clone as 83878-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83878-5-RR (AES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83878-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83878-5-RR

    Affinity and Kinetic Characterization of 83878-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83878-5-RR against Human AES were performed. The affinity constant is 1.76 nM.

AES Recombinant antibody, PBS Only

AES Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83878-5-PBS

产品说明书

CloneNo. 240987D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83878-5-PBS targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AES fusion protein Ag21021 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 amino-terminal enhancer of split
别名 TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2
计算分子量 264 aa, 29 kDa
观测分子量 22-29 kDa
GenBank蛋白编号BC113735
基因名称 AES
Gene ID (NCBI) 166
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ08117
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).