验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells, DU 145 cells, MDA-MB-231 cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83878-5-RR targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | AES fusion protein Ag21021 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | amino-terminal enhancer of split |
| 别名 | TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2 |
| 计算分子量 | 264 aa, 29 kDa |
| 观测分子量 | 22-29 kDa |
| GenBank蛋白编号 | BC113735 |
| 基因名称 | AES |
| Gene ID (NCBI) | 166 |
| RRID | AB_3671462 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q08117 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AES antibody 83878-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |