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验证数据展示

  • WB analysis of HepG2 using 81083-1-RR (same clone as 81083-1-PBS)

    WB analysis of HepG2 using 81083-1-RR (same clone as 81083-1-PBS)

    WB result of AFP antibody (81083-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFP transfected HepG2 cells. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    WB analysis using 81083-1-RR (same clone as 81083-1-PBS)

    human placenta were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    IHC staining of human colon cancer using 81083-1-RR (same clone as 81083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81083-1-RR (AFP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AFP antibody included
  • WB analysis of HuH-7 using 81083-1-RR (same clone as 81083-1-PBS)

    WB analysis of HuH-7 using 81083-1-RR (same clone as 81083-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 81083-1-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81083-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81083-1-RR

    Affinity and Kinetic Characterization of 81083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81083-1-RR against Human AFP were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

AFP Recombinant antibody, PBS Only

AFP Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81083-1-PBS

产品说明书

CloneNo. 2D17

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.

产品信息

81083-1-PBS targets AFP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 AFP fusion protein Ag6089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alpha-fetoprotein
别名 Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin
计算分子量 69 kDa
观测分子量68-72 kDa
GenBank蛋白编号BC027881
基因名称 AFP
Gene ID (NCBI) 174
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP02771
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).