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AFP Recombinant antibody
AFP Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2D17
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HuH-7 cells, human placenta tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81083-1-RR targets AFP in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | AFP fusion protein Ag6089 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | alpha-fetoprotein |
Synonyms | Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin |
Calculated Molecular Weight | 69 kDa |
Observed Molecular Weight | 68-72 kDa |
GenBank Accession Number | BC027881 |
Gene Symbol | AFP |
Gene ID (NCBI) | 174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P02771 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AFP antibody 81083-1-RR | Download protocol |
IHC protocol for AFP antibody 81083-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |