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CoraLite® Plus 750-conjugated AFP Recombinant antibody
AFP Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2D17
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81083-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81083 targets AFP in WB applications and shows reactivity with Human samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human |
| Immunogen | AFP fusion protein Ag6089 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | alpha-fetoprotein |
| Synonyms | AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP |
| Calculated Molecular Weight | 69 kDa |
| Observed Molecular Weight | 68-72 kDa |
| GenBank Accession Number | BC027881 |
| Gene Symbol | AFP |
| Gene ID (NCBI) | 174 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02771 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 AFP antibody CL750-81083 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |