验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AFP Ready-To-Use IHC Kit. AFP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81083-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-81083 targets AFP in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | AFP fusion protein Ag6089 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | alpha-fetoprotein |
| 别名 | AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP |
| 计算分子量 | 69 kDa |
| 观测分子量 | 68-72 kDa |
| GenBank蛋白编号 | BC027881 |
| 基因名称 | AFP |
| Gene ID (NCBI) | 174 |
| RRID | AB_3673723 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02771 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult (PubMed:24120489). AFP is considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker (PubMed: 18669658).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 AFP antibody CL750-81083 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |