AGER/RAGE Monoclonal antibody
AGER/RAGE Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, pig and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3D4G11
验证数据展示
经过测试的应用
| Positive WB detected in | pig lung tissue, rat lung tissue |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy AGER/RAGE Ready-To-Use IHC Kit. AGER/RAGE primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
66833-1-Ig targets AGER/RAGE in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Immunogen | AGER/RAGE fusion protein Ag9657 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | advanced glycosylation end product-specific receptor |
| Synonyms | RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11 |
| Calculated Molecular Weight | 404 aa, 43 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC020669 |
| Gene Symbol | AGER |
| Gene ID (NCBI) | 177 |
| RRID | AB_2882176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15109 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| IHC protocol for AGER/RAGE antibody 66833-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Signal Dapagliflozin alleviates advanced glycation end product induced podocyte injury through AMPK/mTOR mediated autophagy pathway | ||
Mol Immunol S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway. | ||
J Ethnopharmacol Zhongfeng Decoction attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy via regulating the AGE-RAGE signaling pathway |
