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验证数据展示

  • WB analysis of pig lung using 66833-1-Ig

    WB analysis of pig lung using 66833-1-Ig

    pig lung tissue were subjected to SDS PAGE followed by western blot with 66833-1-Ig (AGER antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 66833-1-Ig

    IHC staining of human lung using 66833-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGER/RAGE antibody included
  • IHC staining of human lung using 66833-1-Ig

    IHC staining of human lung using 66833-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66833-1-Ig (AGER antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGER/RAGE antibody included
  • IF Staining of mouse lung using 66833-1-Ig

    IF Staining of mouse lung using 66833-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat lung using 66833-1-Ig

    IF Staining of rat lung using 66833-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AGER antibody (66833-1-Ig, Clone: 3D4G11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AGER/RAGE Monoclonal antibody

AGER/RAGE Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66833-1-Ig

产品说明书

CloneNo. 3D4G11
引用文献(3)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig lung tissue, rat lung tissue
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat lung tissue, mouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy AGER/RAGE Ready-To-Use IHC Kit. AGER/RAGE primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

66833-1-Ig targets AGER/RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat
免疫原 AGER/RAGE fusion protein Ag9657 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11
计算分子量 404 aa, 43 kDa
观测分子量 45 kDa
GenBank蛋白编号BC020669
基因名称 AGER
Gene ID (NCBI) 177
RRIDAB_2882176
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15109
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

实验方案

Product Specific Protocols
WB protocol for AGER/RAGE antibody 66833-1-IgDownload protocol
IHC protocol for AGER/RAGE antibody 66833-1-IgDownload protocol
IF protocol for AGER/RAGE antibody 66833-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Signal

Dapagliflozin alleviates advanced glycation end product induced podocyte injury through AMPK/mTOR mediated autophagy pathway

Authors - Lei Yang
View Article
humanWB

Mol Immunol

S100A11 regulates nasal epithelial cell remodeling and inflammation in CRSwNPs via the RAGE-mediated AMPK-STAT3 pathway.

Authors - Chengcheng Liu
View Article
ratWB

J Ethnopharmacol

Zhongfeng Decoction attenuates cerebral ischemia-reperfusion injury by inhibiting autophagy via regulating the AGE-RAGE signaling pathway

Authors - Weitao Wang
View Article