• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using CL488-66833

    WB analysis using CL488-66833

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66833 (AGER antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of rat lung using CL488-66833

    IF Staining of rat lung using CL488-66833

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using CoraLite® Plus 488 AGER antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated AGER/RAGE Monoclonal antibody

AGER/RAGE Monoclonal Antibody for WB, IF-P
Cat No. CL488-66833

产品说明书

CloneNo. 3D4G11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IF-P

别名

RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig lung tissue
Positive IF-P detected inrat lung tissue
Planning an IHC experiment? We recommend our IHCeasy AGER/RAGE Ready-To-Use IHC Kit. AGER/RAGE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66833-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66833 targets AGER/RAGE in WB, IF-P applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IF-P Application Description
经测试反应性 human, rat, pig
免疫原 AGER/RAGE fusion protein Ag9657 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11
计算分子量 404 aa, 43 kDa
观测分子量 43, 50 kDa
GenBank蛋白编号BC020669
基因名称 AGER
Gene ID (NCBI) 177
RRIDAB_3672924
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15109
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 AGER/RAGE antibody CL488-66833Download protocol
IF protocol for CL Plus 488 AGER/RAGE antibody CL488-66833Download protocol
Standard Protocols
Click here to view our Standard Protocols