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CoraLite® Plus 488-conjugated AGER/RAGE Monoclonal antibody

AGER/RAGE Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D4G11

Cat no : CL488-66833

Print datasheet

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11

验证数据展示

  • IF Staining of rat lung using CL488-66833

    IF Staining of rat lung using CL488-66833

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using CoraLite® Plus 488 AGER antibody (CL488-66833, Clone: 3D4G11 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inrat lung tissue
Planning an IHC experiment? We recommend our IHCeasy AGER/RAGE Ready-To-Use IHC Kit. AGER/RAGE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66833-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66833 targets AGER/RAGE in IF-P applications and shows reactivity with human, rat, pig samples.

Tested Applications IF-P Application Description
Tested Reactivity human, rat, pig
Immunogen AGER/RAGE fusion protein Ag9657 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name advanced glycosylation end product-specific receptor
Synonyms RAGE, AGER, Advanced glycosylation end product-specific receptor, 3D4G11
Calculated Molecular Weight 404 aa, 43 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC020669
Gene Symbol AGER
Gene ID (NCBI) 177
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15109
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a multiligand receptor of the immunoglobulin superfamily. It interacts with distinct molecules implicated in homeostasis, development, and inflammation, and certain diseases such as diabetes and Alzheimer disease.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AGER/RAGE antibody CL488-66833Download protocol
Standard Protocols
Click here to view our Standard Protocols