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AGGF1 Recombinant antibody

AGGF1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242264E9

Cat no : 84715-3-RR

Print datasheet

Synonyms

HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7, Angiogenic factor VG5Q

验证数据展示

  • WB analysis using 84715-3-RR

    WB analysis using 84715-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84715-3-RR (AGGF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84715-3-RR

    Affinity and Kinetic Characterization of 84715-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84715-3-RR against Human AGGF1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HCT 116 cells, HeLa cells, L02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84715-3-RR targets AGGF1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen AGGF1 fusion protein Ag2497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name angiogenic factor with G patch and FHA domains 1
Synonyms HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7, Angiogenic factor VG5Q
Calculated Molecular Weight 714 aa, 81 kDa
Observed Molecular Weight 84-100 kDa
GenBank Accession NumberBC029382
Gene Symbol AGGF1
Gene ID (NCBI) 55109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8N302
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.

实验方案

Product Specific Protocols
WB protocol for AGGF1 antibody 84715-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols