验证数据展示
经过测试的应用
| Positive WB detected in | human plasma |
| Positive IHC detected in | human liver tissue, human hepatocirrhosis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HepG2 cells, L02 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
23972-1-AP targets Angiotensinogen/AGT in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Immunogen | Angiotensinogen/AGT fusion protein Ag21121 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Synonyms | AGT, Angiotensinogen, Serpin A8, SERPINA8, Ang IV |
| Calculated Molecular Weight | 485 aa, 53 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC011519 |
| Gene Symbol | AGT |
| Gene ID (NCBI) | 183 |
| RRID | AB_2879383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P01019 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Angiotensinogen/AGT antibody 23972-1-AP | Download protocol |
| IHC protocol for Angiotensinogen/AGT antibody 23972-1-AP | Download protocol |
| IF protocol for Angiotensinogen/AGT antibody 23972-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
