CoraLite® Plus 488-conjugated Angiotensinogen Polyclonal antibody
Angiotensinogen Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AGT Ready-To-Use IHC Kit. AGT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 23972-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-23972 targets Angiotensinogen in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | Angiotensinogen fusion protein Ag21121 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Synonyms | AGT, Serpin A8, SERPINA8, Ang IV, Ang III |
| Calculated Molecular Weight | 485 aa, 53 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC011519 |
| Gene Symbol | AGT |
| Gene ID (NCBI) | 183 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01019 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Angiotensinogen antibody CL488-23972 | Download protocol |
| FC protocol for CL Plus 488 Angiotensinogen antibody CL488-23972 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
