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AGXT Recombinant antibody

AGXT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240305E7

Cat no : 83385-6-RR

Print datasheet

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

验证数据展示

  • WB analysis using 83385-6-RR

    WB analysis using 83385-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83385-6-RR

    WB analysis using 83385-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83385-6-RR

    FC experiment of HepG2 using 83385-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 83385-6-RR

    IF Staining of HeLa using 83385-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83385-6-RR

    Affinity and Kinetic Characterization of 83385-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, HuH-7 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83385-6-RR is AGXT Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen AGXT fusion protein Ag18062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
WB protocol for AGXT antibody 83385-6-RRDownload protocol
IF protocol for AGXT antibody 83385-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols