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验证数据展示

  • WB analysis using 83385-6-RR

    WB analysis using 83385-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83385-6-RR

    WB analysis using 83385-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83385-6-RR

    FC experiment of HepG2 using 83385-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 83385-6-RR

    IF Staining of HeLa using 83385-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83385-6-RR

    Affinity and Kinetic Characterization of 83385-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

AGXT Recombinant antibody

AGXT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83385-6-RR
CloneNo. 240305E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, HuH-7 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83385-6-RR targets AGXT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen AGXT fusion protein Ag18062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
RRIDAB_3671041
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
WB protocol for AGXT antibody 83385-6-RRDownload protocol
IF protocol for AGXT antibody 83385-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols