CoraLite® Plus 488-conjugated AGXT Recombinant antibody
AGXT Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240305E7
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83385-6-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83385-6 is AGXT Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human |
| Immunogen | AGXT fusion protein Ag18062 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | alanine-glyoxylate aminotransferase |
| Synonyms | EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT |
| Calculated Molecular Weight | 392 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC132819 |
| Gene Symbol | AGXT |
| Gene ID (NCBI) | 189 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P21549 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AGXT antibody CL488-83385-6 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |