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CoraLite® Plus 488-conjugated AGXT Recombinant antibody

AGXT Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240305E7

Cat No : CL488-83385-6

Print datasheet

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

验证数据展示

  • IF Staining of HeLa using CL488-83385-6

    IF Staining of HeLa using CL488-83385-6

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AGXT antibody (CL488-83385-6, Clone: 240305E7 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83385-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83385-6 is AGXT Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen AGXT fusion protein Ag18062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21549
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AGXT antibody CL488-83385-6Download protocol
Standard Protocols
Click here to view our Standard Protocols