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AHR Monoclonal antibody

AHR Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67785-1-Ig

产品说明书

CloneNo. 2D1F9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HEK-293 cells, HSC-T6 cells, 4T1 cells, PC-3 cells, HeLa cells, Caco-2 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, mouse testis tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67785-1-Ig targets AHR in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 AHR fusion protein Ag28935 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9
计算分子量 848 aa, 96 kDa
观测分子量105-110 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
RRIDAB_2918549
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35869
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
WB protocol for AHR antibody 67785-1-IgDownload protocol
IHC protocol for AHR antibody 67785-1-IgDownload protocol
IF protocol for AHR antibody 67785-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Phytother Res

Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity

Authors - Xiaojing Wang
mouseWB

NPJ Regen Med

Aryl hydrocarbon receptor regulates IL-22 receptor expression on thymic epithelial cell and accelerates thymus regeneration

Authors - Jingyi Shen
mouseIF

Ecotoxicol Environ Saf

Hesperetin protects hippocampal neurons from the neurotoxicity of Aflatoxin B1 in mice

Authors - Chao Song
ratWB

Biomater Sci

Glioma-targeted multifunctional nanoparticles to co-deliver camptothecin and curcumin for enhanced chemo-immunotherapy.

Authors - Zian Wang
mouseWB,IF

iScience

StemRegenin 1 attenuates the RANKL-induced osteoclastogenesis via inhibiting AhR-c-src-NF-κB/p-ERK MAPK-NFATc1 signaling pathway

Authors - Shengji Zhou
human,mouseWB,IF,FC

J Inflamm Res

MBL Binding with AhR Controls Th17 Immunity in Silicosis-Associated Lung Inflammation and Fibrosis

Authors - Yunzhi Liu
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