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  • Featured Product
  • KD/KO Validated

AHR Monoclonal antibody, PBS Only

AHR Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2D1F9

Cat No : 67785-1-PBS

Print datasheet

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

验证数据展示

  • WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHR antibody included
  • IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHR antibody included
  • IF Staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    IF Staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Blue (DAPI). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 67785-1-Ig (same clone as 67785-1-PBS)

    IF Staining of mouse testis using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

产品信息

67785-1-PBS targets AHR in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen AHR fusion protein Ag28935 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name aryl hydrocarbon receptor
Synonyms Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35869
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)