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验证数据展示

  • IF Staining of A431 using CL488-28727

    IF Staining of A431 using CL488-28727

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 AHR antibody (CL488-28727) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AHR Polyclonal antibody

AHR Polyclonal Antibody for IF/ICC
Cat No. CL488-28727

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC

Synonyms

AHR, bHLHe76, Class E basic helix-loop-helix protein 76, Ah receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28727-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28727 targets AHR in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen AHR fusion protein Ag28935 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aryl hydrocarbon receptor
Synonyms AHR, bHLHe76, Class E basic helix-loop-helix protein 76, Ah receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
RRIDAB_3672833
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35869
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AHR antibody CL488-28727Download protocol
Standard Protocols
Click here to view our Standard Protocols