验证数据展示
经过测试的应用
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-28727 targets AHR in IF/ICC applications and shows reactivity with Human samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human |
免疫原 | AHR fusion protein Ag28935 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | aryl hydrocarbon receptor |
别名 | AHR, bHLHe76, Class E basic helix-loop-helix protein 76, Ah receptor |
计算分子量 | 848 aa, 96 kDa |
观测分子量 | 105-110 kDa |
GenBank蛋白编号 | BC070080 |
基因名称 | AHR |
Gene ID (NCBI) | 196 |
RRID | AB_3672833 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P35869 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 AHR antibody CL488-28727 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |