• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HepG2 using CL488-67785

    FC experiment of HepG2 using CL488-67785

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human AHR (CL488-67785, Clone:2D1F9) (red), or 0.8 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AHR Monoclonal antibody

AHR Monoclonal Antibody for FC (Intra)
Cat No. CL488-67785

产品说明书

CloneNo. 2D1F9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

FC (Intra)

别名

AHR, bHLHe76, Class E basic helix-loop-helix protein 76, 2D1F9, Ah receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67785-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67785 targets AHR in FC (Intra) applications and shows reactivity with Human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, rat
免疫原 AHR fusion protein Ag28935 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 AHR, bHLHe76, Class E basic helix-loop-helix protein 76, 2D1F9, Ah receptor
计算分子量 848 aa, 96 kDa
观测分子量105-110 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35869
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 AHR antibody CL488-67785Download protocol
Standard Protocols
Click here to view our Standard Protocols