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验证数据展示

  • FC experiment of HepG2 using CL647-67785

    FC experiment of HepG2 using CL647-67785

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human AHR (CL647-67785, Clone:2D1F9) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated AHR Monoclonal antibody

AHR Monoclonal Antibody for
Cat No. CL647-67785
CloneNo. 2D1F9

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

Synonyms

AHR, bHLHe76, Class E basic helix-loop-helix protein 76, 2D1F9, Ah receptor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  {{ptg:cur_Conjugation}}
Size: 

-/ -

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经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67785-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67785 targets AHR in applications and shows reactivity with Human samples.

Tested Applications Application Description
Tested Reactivity Human
Immunogen AHR fusion protein Ag28935 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name aryl hydrocarbon receptor
Synonyms AHR, bHLHe76, Class E basic helix-loop-helix protein 76, 2D1F9, Ah receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35869
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 AHR antibody CL647-67785Download protocol
Standard Protocols
Click here to view our Standard Protocols