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验证数据展示

  • FC experiment of K-562 using CL488-68049

    FC experiment of K-562 using CL488-68049

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AIFM2 (CL488-68049, Clone:1A2B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated AIFM2/ FSP1 Monoclonal antibody

AIFM2/ FSP1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-68049

产品说明书

CloneNo. 1A2B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

FC (Intra)

别名

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inK-562 cells
Positive FC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68049-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68049 targets AIFM2/ FSP1 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse
免疫原 AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 2
别名 AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC023601
基因名称 AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2923870
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRQ8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 AIFM2/ FSP1 antibody CL488-68049Download protocol
Standard Protocols
Click here to view our Standard Protocols