CoraLite® Plus 488-conjugated AIFM2/ FSP1 Monoclonal antibody
AIFM2/ FSP1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A2B2
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | K-562 cells |
Positive FC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68049-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68049 targets AIFM2/ FSP1 in FC (Intra) applications and shows reactivity with Human, Mouse samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | Human, Mouse |
Immunogen | AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | apoptosis-inducing factor, mitochondrion-associated, 2 |
Synonyms | AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 41 kDa |
GenBank Accession Number | BC023601 |
Gene Symbol | AIFM2/ FSP1 |
Gene ID (NCBI) | 84883 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9BRQ8 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 AIFM2/ FSP1 antibody CL488-68049 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |