验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82974-1-RR targets AIFM2/ FSP1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, FC (Intra), IP, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | AIFM2/ FSP1 fusion protein Ag13412 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | apoptosis-inducing factor, mitochondrion-associated, 2 |
| Synonyms | AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC023601 |
| Gene Symbol | AIFM2/ FSP1 |
| Gene ID (NCBI) | 84883 |
| RRID | AB_3670722 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BRQ8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
| IP protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
| FC protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |