验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82974-1-RR targets AIFM2/ FSP1 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC (Intra), IP, ELISA Application Description |
经测试反应性 | human |
免疫原 | AIFM2/ FSP1 fusion protein Ag13412 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | apoptosis-inducing factor, mitochondrion-associated, 2 |
别名 | AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2 |
计算分子量 | 41 kDa |
观测分子量 | 41 kDa |
GenBank蛋白编号 | BC023601 |
基因名称 | AIFM2/ FSP1 |
Gene ID (NCBI) | 84883 |
RRID | AB_3670722 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9BRQ8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.
实验方案
Product Specific Protocols | |
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WB protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
IP protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
FC protocol for AIFM2/ FSP1 antibody 82974-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |