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验证数据展示

  • WB analysis using 66902-3-Ig

    WB analysis using 66902-3-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66902-3-Ig (AIM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB, ELISA
Cat No. 66902-3-Ig

Print datasheet

CloneNo. 4C6E1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

PYHIN4, Interferon-inducible protein AIM2, 4C6E1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Karpas-299 cells, U266 cells, MJ cells, HuT 78 cells, U-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66902-3-Ig targets AIM2 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen AIM2 fusion protein Ag14607 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name absent in melanoma 2
Synonyms PYHIN4, Interferon-inducible protein AIM2, 4C6E1
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
RRIDAB_3670376
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14862
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for AIM2 antibody 66902-3-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols