验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, Raji cells, Karpas-299 cells, U266 cells, MJ cells, HuT 78 cells, U-87 MG cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66902-3-Ig targets AIM2 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | AIM2 fusion protein Ag14607 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | absent in melanoma 2 |
别名 | PYHIN4, Interferon-inducible protein AIM2, 4C6E1 |
计算分子量 | 343 aa, 39 kDa |
观测分子量 | 39 kDa |
GenBank蛋白编号 | BC010940 |
基因名称 | AIM2 |
Gene ID (NCBI) | 9447 |
RRID | AB_3670376 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O14862 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AIM2 antibody 66902-3-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |