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CoraLite® Plus 488-conjugated AIRE Monoclonal antibody

AIRE Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3C3

Cat no : CL488-66262

Print datasheet

Synonyms

PGA1, APSI, APS1, APECED, AIRE1

验证数据展示

  • FC experiment of HeLa using CL488-66262

    FC experiment of HeLa using CL488-66262

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AIRE (CL488-66262, Clone:1A3C3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-66262

    IF Staining of HeLa using CL488-66262

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 AIRE antibody (CL488-66262, Clone: 1A3C3 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIRE Ready-To-Use IHC Kit. AIRE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66262-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66262 targets AIRE in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, pig
Immunogen AIRE fusion protein Ag19103 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name autoimmune regulator
Synonyms PGA1, APSI, APS1, APECED, AIRE1
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43918
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AIRE antibody CL488-66262Download protocol
FC protocol for CL Plus 488 AIRE antibody CL488-66262Download protocol
Standard Protocols
Click here to view our Standard Protocols