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验证数据展示

  • WB analysis of MCF-7 using 13206-1-AP

    WB analysis of MCF-7 using 13206-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 13206-1-AP

    IHC staining of human breast cancer using 13206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AK4/AK3L1 antibody included
  • IHC staining of human breast cancer using 13206-1-AP

    IHC staining of human breast cancer using 13206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AK4/AK3L1 antibody included
  • IP experiment of MCF-7 using 13206-1-AP

    IP experiment of MCF-7 using 13206-1-AP

    IP result of anti-AK3L1 (IP:13206-1-AP, 4ug; Detection:13206-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

  • IF Staining of MCF-7 using 13206-1-AP

    IF Staining of MCF-7 using 13206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13206-1-AP (AK3L1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

AK3L1 Polyclonal antibody

AK3L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13206-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA

别名

AK4, AK3L 1, AK3, AK 4, Adenylate kinase 4, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, human liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy AK4/AK3L1 Ready-To-Use IHC Kit. AK4/AK3L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13206-1-AP targets AK3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原 AK3L1 fusion protein Ag3966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 3-like 1
别名 AK4, AK3L 1, AK3, AK 4, Adenylate kinase 4, mitochondrial
计算分子量 223 aa, 25 kDa
观测分子量 30 kDa
GenBank蛋白编号BC040224
基因名称 AK3L1
Gene ID (NCBI) 205
RRIDAB_2225456
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

实验方案

Product Specific Protocols
WB protocol for AK3L1 antibody 13206-1-APDownload protocol
IHC protocol for AK3L1 antibody 13206-1-APDownload protocol
IF protocol for AK3L1 antibody 13206-1-APDownload protocol
IP protocol for AK3L1 antibody 13206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.

Authors - Chunbao Zang
  • KD Validated
View Article
ratWB,IHC

Exp Brain Res

MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage

Authors - Wei Jiang
View Article