验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, human liver tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AK4/AK3L1 Ready-To-Use IHC Kit. AK4/AK3L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3600 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
13206-1-AP targets AK3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Immunogen | AK3L1 fusion protein Ag3966 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | adenylate kinase 3-like 1 |
| Synonyms | AK4, AK3L 1, AK3, AK 4, Adenylate kinase 4, mitochondrial |
| Calculated Molecular Weight | 223 aa, 25 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC040224 |
| Gene Symbol | AK3L1 |
| Gene ID (NCBI) | 205 |
| RRID | AB_2225456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P27144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IHC protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IF protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| IP protocol for AK3L1 antibody 13206-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.
| ||
Exp Brain Res MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage |
