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验证数据展示

  • IF Staining of MCF-7 using CL488-13206

    IF Staining of MCF-7 using CL488-13206

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 AK3L1 antibody (CL488-13206) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AK3L1 Polyclonal antibody

AK3L1 Polyclonal Antibody for IF/ICC
Cat No. CL488-13206

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC

别名

Adenylate kinase 3 like, adenylate kinase 3 like 1, AK3, AK3L1, AK4, ATP AMP transphosphorylase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy AK4/AK3L1 Ready-To-Use IHC Kit. AK4/AK3L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13206-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13206 targets AK3L1 in IF/ICC applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse
免疫原 AK3L1 fusion protein Ag3966 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adenylate kinase 3-like 1
别名 Adenylate kinase 3 like, adenylate kinase 3 like 1, AK3, AK3L1, AK4, ATP AMP transphosphorylase
计算分子量 223 aa, 25 kDa
观测分子量 30 kDa
GenBank蛋白编号BC040224
基因名称 AK3L1
Gene ID (NCBI) 205
RRIDAB_3672560
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP27144
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AK3L1 antibody CL488-13206Download protocol
Standard Protocols
Click here to view our Standard Protocols