• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

    WB analysis using 85119-2-RR (same clone as 85119-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

    IF Staining of HepG2 using 85119-2-RR (same clone as 85119-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

    FC experiment of HEK-293T using 85119-2-RR (same clone as 85119-2-PBS)

    1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85119-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01840-1

    Cytometric bead array standard curve of MP01840-1

    Cytometric bead array standard curve of MP01840-1, AKAP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85119-3-PBS. Detection antibody: 85119-2-PBS. Standard: Ag8037. Range: 0.781-100 ng/mL.

AKAP1 Recombinant antibody, PBS Only (Detector)

AKAP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 85119-2-PBS

产品说明书

CloneNo. 242763A5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

85119-2-PBS targets AKAP1 as part of a matched antibody pair:

MP01840-1: 85119-3-PBS capture and 85119-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 AKAP1 fusion protein Ag8037 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 1
别名 242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa
计算分子量 97 kDa
观测分子量149 kDa
GenBank蛋白编号BC000729
基因名称 AKAP1
Gene ID (NCBI) 8165
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92667
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.