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Conjugate
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AKAP3 Recombinant antibody

AKAP3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230278C3

Cat no : 82986-1-RR

Print datasheet

Synonyms

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

验证数据展示

  • WB analysis using 82986-1-RR

    WB analysis using 82986-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82986-1-RR (AKAP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82986-1-RR

    Affinity and Kinetic Characterization of 82986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82986-1-RR against Human AKAP3 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82986-1-RR targets AKAP3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AKAP3 fusion protein Ag33655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name A kinase (PRKA) anchor protein 3
Synonyms AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75969
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

实验方案

Product Specific Protocols
WB protocol for AKAP3 antibody 82986-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols