验证数据展示
经过测试的应用
| Positive WB detected in | rat testis tissue |
For other applications, we recommend the unconjugated version of this antibody, 82986-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-82986 targets AKAP3 in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | AKAP3 fusion protein Ag33655 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | A kinase (PRKA) anchor protein 3 |
| 别名 | AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3 |
| 计算分子量 | 95 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC047535 |
| 基因名称 | AKAP3 |
| Gene ID (NCBI) | 10566 |
| RRID | AB_3673758 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O75969 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 AKAP3 antibody CL750-82986 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |