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CoraLite® Plus 750-conjugated AKAP3 Recombinant antibody

AKAP3 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230278C3

Cat No : CL750-82986

Print datasheet

Synonyms

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

验证数据展示

  • WB analysis using CL750-82986

    WB analysis using CL750-82986

    Rat testis was subjected to SDS PAGE followed by western blot with CL750-82986 (AKAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inrat testis tissue
For other applications, we recommend the unconjugated version of this antibody, 82986-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82986 targets AKAP3 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen AKAP3 fusion protein Ag33655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name A kinase (PRKA) anchor protein 3
Synonyms AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75969
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 AKAP3 antibody CL750-82986Download protocol
Standard Protocols
Click here to view our Standard Protocols