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CoraLite® Plus 488-conjugated AKAP3 Monoclonal antibody

AKAP3 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11H9

Cat no : CL488-66255

Print datasheet

Synonyms

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

验证数据展示

  • IF Staining of mouse testis using CL488-66255

    IF Staining of mouse testis using CL488-66255

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 AKAP3 antibody (CL488-66255, Clone: 3C11H9 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-66255

    FC experiment of Jurkat using CL488-66255

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AKAP3 Monoclonal antibody (CL488-66255, Clone:3C11H9)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66255-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66255 targets AKAP3 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen AKAP3 fusion protein Ag4885 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 3
Synonyms AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75969
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AKAP3 antibody CL488-66255Download protocol
FC protocol for CL Plus 488 AKAP3 antibody CL488-66255Download protocol
Standard Protocols
Click here to view our Standard Protocols