• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of mouse testis using CL488-66255

    IF Staining of mouse testis using CL488-66255

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 AKAP3 antibody (CL488-66255, Clone: 3C11H9 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL488-66255

    FC experiment of Jurkat using CL488-66255

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated AKAP3 Monoclonal antibody (CL488-66255, Clone:3C11H9)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated AKAP3 Monoclonal antibody

AKAP3 Monoclonal Antibody for IF-P
Cat No. CL488-66255

产品说明书

CloneNo. 3C11H9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

IF-P

别名

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66255-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66255 targets AKAP3 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 AKAP3 fusion protein Ag4885 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 3
别名 AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3
计算分子量 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC047535
基因名称 AKAP3
Gene ID (NCBI) 10566
RRIDAB_3084225
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75969
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AKAP3 antibody CL488-66255Download protocol
FC protocol for CL Plus 488 AKAP3 antibody CL488-66255Download protocol
Standard Protocols
Click here to view our Standard Protocols