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Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

ALCAM Uni-rAbTM Recombinant Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

3A6

Cat no : 65611-1-MR

Print datasheet

Synonyms

MEMD, CD166, 3A6

验证数据展示

  • IF Staining of hTERT-RPE1 using 65611-1-MR

    IF Staining of hTERT-RPE1 using 65611-1-MR

    Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of human PBMCs using 65611-1-MR

    FC experiment of human PBMCs using 65611-1-MR

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed.

经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65611-1-MR targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human
Immunogen Cultured human thymic epithelial cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms MEMD, CD166, 3A6
Calculated Molecular Weight 105 kDa
GenBank Accession NumberBC057809
Gene Symbol ALCAM
Gene ID (NCBI) 214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
IF protocol for ALCAM antibody 65611-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols