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验证数据展示

  • IF Staining of hTERT-RPE1 using 65611-1-MR

    IF Staining of hTERT-RPE1 using 65611-1-MR

    Immunofluorescent analysis of un-fixed hTERT-RPE1 cells using ALCAM antibody (65611-1-MR, Clone: 3A6 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of human PBMCs using 65611-1-MR

    FC experiment of human PBMCs using 65611-1-MR

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Untreated human PBMCs were surface stained with 0.25 ug Anti-Human ALCAM Mouse IgG2a Recombinant Antibody (65611-1-MR, Clone: 3A6) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) (black). Cells were not fixed.

Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody

ALCAM Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 65611-1-MR

产品说明书

CloneNo. 3A6

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC, FC

别名

MEMD, CD166, 3A6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65611-1-MR targets ALCAM in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Cultured human thymic epithelial cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 MEMD, CD166, 3A6
计算分子量 105 kDa
GenBank蛋白编号BC057809
基因名称 ALCAM
Gene ID (NCBI) 214
RRIDAB_3670358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
IF protocol for ALCAM antibody 65611-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols