CoraLite® Plus 647 Anti-Human ALCAM (3A6) Mouse IgG2a Recombinant Antibody
ALCAM Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3A6
验证数据展示
经过测试的应用
Positive FC detected in | PHA treated human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65611-1-MR
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65611 targets ALCAM in FC applications and shows reactivity with human samples.
Tested Applications | FC Application Description |
Tested Reactivity | human |
Immunogen | Cultured human thymic epithelial cells 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Full Name | activated leukocyte cell adhesion molecule |
Synonyms | MEMD, CD166, 3A6 |
Calculated Molecular Weight | 105 kDa |
GenBank Accession Number | BC057809 |
Gene Symbol | ALCAM |
Gene ID (NCBI) | 214 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. |
背景介绍
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 ALCAM antibody CL647-65611 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |