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ALCAM Monoclonal antibody

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F7D6

Cat no : 67768-1-Ig

Print datasheet

Synonyms

MEMD, CD166, 1F7D6

验证数据展示

  • WB analysis using 67768-1-Ig

    WB analysis using 67768-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 67768-1-Ig

    IF Staining of hTERT-RPE1 using 67768-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • IHC staining of human breast cancer using 67768-1-Ig

    IHC staining of human breast cancer using 67768-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67768-1-Ig

    IHC staining of human breast cancer using 67768-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human breast cancer using 67768-1-Ig

    IF Staining of human breast cancer using 67768-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human breast cancer using 67768-1-Ig

    IF Staining of human breast cancer using 67768-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHT-1080 cells, U2OS cells, A549 cells, LNCaP cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67768-1-Ig targets ALCAM in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ALCAM fusion protein Ag16892 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms MEMD, CD166, 1F7D6
Calculated Molecular Weight 583 aa, 65 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC137097
Gene Symbol ALCAM
Gene ID (NCBI) 214
RRIDAB_2918534
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13740
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
WB protocol for ALCAM antibody 67768-1-IgDownload protocol
IHC protocol for ALCAM antibody 67768-1-IgDownload protocol
IF protocol for ALCAM antibody 67768-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Antiviral Res

Harnessing host enhancers of SARS-CoV-2 entry as novel targets for antiviral therapy

Authors - Nathalia Williams
View Article