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ALCAM Monoclonal antibody, PBS Only

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7D6

Cat no : 67768-1-PBS

Print datasheet

Synonyms

MEMD, CD166, 1F7D6

验证数据展示

  • WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

产品信息

67768-1-PBS targets ALCAM in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ALCAM fusion protein Ag16892 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms MEMD, CD166, 1F7D6
Calculated Molecular Weight 583 aa, 65 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC137097
Gene Symbol ALCAM
Gene ID (NCBI) 214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13740
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).