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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

ALCAM Monoclonal antibody, PBS Only

ALCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA
Cat No. 67768-1-PBS

产品说明书

CloneNo. 1F7D6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

别名

MEMD, CD166, 1F7D6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67768-1-PBS targets ALCAM in WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA Application Description
经测试反应性 human
免疫原 ALCAM fusion protein Ag16892 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 activated leukocyte cell adhesion molecule
别名 MEMD, CD166, 1F7D6
计算分子量 583 aa, 65 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC137097
基因名称 ALCAM
Gene ID (NCBI) 214
RRIDAB_2918534
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13740
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).