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ALCAM Monoclonal antibody, PBS Only

ALCAM Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1F7D6

Cat No : 67768-1-PBS

Print datasheet

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

验证数据展示

  • WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

产品信息

67768-1-PBS targets ALCAM in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen ALCAM fusion protein Ag16892 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms ALCAM, CD166, CD166 antigen, FLJ38514, MEMD
Calculated Molecular Weight 583 aa, 65 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC137097
Gene Symbol ALCAM
Gene ID (NCBI) 214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13740
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).