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验证数据展示

  • WB analysis of HeLa using 22220-1-AP

    WB analysis of HeLa using 22220-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 22220-1-AP

    IF Staining of MCF-7 using 22220-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ALDH1B1 antibody 22220-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human liver using 22220-1-AP

    IHC staining of human liver using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of rat kidney using 22220-1-AP

    IHC staining of rat kidney using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of rat kidney using 22220-1-AP

    IHC staining of rat kidney using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human colon cancer using 22220-1-AP

    IHC staining of human colon cancer using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human colon cancer using 22220-1-AP

    IHC staining of human colon cancer using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included
  • IHC staining of human ovary tumor using 22220-1-AP

    IHC staining of human ovary tumor using 22220-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22220-1-AP (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH1B1 antibody included

ALDH1B1 Polyclonal antibody

ALDH1B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22220-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, rat brain tissue
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue, human liver tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ALDH1B1 Ready-To-Use IHC Kit. ALDH1B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22220-1-AP targets ALDH1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原 ALDH1B1 fusion protein Ag17557 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member B1
别名 EC:1.2.1.3, ALDHX, ALDH5, Aldehyde dehydrogenase X, mitochondrial, Aldehyde dehydrogenase 5
计算分子量 517 aa, 57 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC001619
基因名称 ALDH1B1
Gene ID (NCBI) 219
RRIDAB_2879035
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30837
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112). This antibody is specific to ALDH1B1.

实验方案

Product Specific Protocols
WB protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IHC protocol for ALDH1B1 antibody 22220-1-APDownload protocol
IF protocol for ALDH1B1 antibody 22220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Oncotarget

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Authors - Hao-Long Zeng
View Article
humanIHC

Transl Cancer Res

ALDH1B1 predicts poor survival for locally advanced nasopharyngeal carcinoma patients.

Authors - Tao Zhu
View Article