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CoraLite® Plus 488-conjugated ALDH1L1 Polyclonal antibody

ALDH1L1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-17390

Print datasheet

Synonyms

10 FTHFDH, ALDH1L1, FTHFD

验证数据展示

  • IF Staining of mouse brain using CL488-17390

    IF Staining of mouse brain using CL488-17390

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 ALDH1L1 antibody (CL488-17390) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 17390-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17390 targets ALDH1L1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen ALDH1L1 fusion protein Ag11285 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 1 family, member L1
Synonyms 10 FTHFDH, ALDH1L1, FTHFD
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC027241
Gene Symbol ALDH1L1
Gene ID (NCBI) 10840
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75891
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ALDH1L1 antibody CL488-17390Download protocol
Standard Protocols
Click here to view our Standard Protocols