验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rabbit liver tissue, rat liver tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
68237-1-Ig targets ALDH2 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat, Rabbit, Pig |
| Cited Reactivity | mouse |
| Immunogen | ALDH2 fusion protein Ag7463 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | aldehyde dehydrogenase 2 family (mitochondrial) |
| Synonyms | ALDH class 2, ALDH E2, ALDH2, ALDHI, ALDM |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002967 |
| Gene Symbol | ALDH2 |
| Gene ID (NCBI) | 217 |
| RRID | AB_2935324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05091 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH2 antibody 68237-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Arch Biochem Biophys Inhibiting apoptosis and GSDME-mediated pyroptosis attenuates hepatic injury in septic mice | ||
Mol Nutr Food Res Protective Effect and Mechanism of L-Theanine on Acute Alcoholic Liver Injury in Mice |