验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293T cells |
| Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15578-1-AP targets ALDH3A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit |
| 免疫原 | ALDH3A1 fusion protein Ag7977 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldehyde dehydrogenase 3 family, memberA1 |
| 别名 | EC:1.2.1.5, ALDHIII, ALDH3, Aldehyde dehydrogenase, dimeric NADP-preferring, AL3A1 |
| 计算分子量 | 50 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC004370 |
| 基因名称 | ALDH3A1 |
| Gene ID (NCBI) | 218 |
| RRID | AB_2273745 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30838 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALDH3A1 antibody 15578-1-AP | Download protocol |
| IHC protocol for ALDH3A1 antibody 15578-1-AP | Download protocol |
| IF protocol for ALDH3A1 antibody 15578-1-AP | Download protocol |
| IP protocol for ALDH3A1 antibody 15578-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats | ||
Adv Healthc Mater Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling. | ||
Biofabrication Development of a novel bioartificial cornea using 3D bioprinting based on electrospun micro-nanofibrous decellularized extracellular matrix | ||
Cell Chem Biol Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons. |