• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ALDH3A1 Polyclonal antibody

ALDH3A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15578-1-AP

Print datasheet

Synonyms

EC:1.2.1.5, ALDHIII, ALDH3, Aldehyde dehydrogenase, dimeric NADP-preferring, AL3A1

验证数据展示

  • WB analysis of A549 using 15578-1-AP

    WB analysis of A549 using 15578-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293T using 15578-1-AP

    IF Staining of HEK-293T using 15578-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using ALDH3A1 antibody (15578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of A549 using 15578-1-AP

    WB analysis of A549 using 15578-1-AP

    WB result of ALDH3A1 antibody (15578-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

  • FC experiment of HEK-293 using 15578-1-AP

    FC experiment of HEK-293 using 15578-1-AP

    1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ALDH3A1 (15578-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of A549 using 15578-1-AP

    WB analysis of A549 using 15578-1-AP

    WB result of ALDH3A1 antibody (15578-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

  • IP experiment of A549 using 15578-1-AP

    IP experiment of A549 using 15578-1-AP

    IP result of anti-ALDH3A1 (IP:15578-1-AP, 4ug; Detection:15578-1-AP 1:3000) with A549 cells lysate 1480 ug.

  • IHC staining of human skin cancer using 15578-1-AP

    IHC staining of human skin cancer using 15578-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15578-1-AP (ALDH3A1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH3A1 antibody included
  • IHC staining of human stomach using 15578-1-AP

    IHC staining of human stomach using 15578-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15578-1-AP (ALDH3A1 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH3A1 antibody included

经过测试的应用

Positive WB detected inA549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293T cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ALDH3A1 Ready-To-Use IHC Kit. ALDH3A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15578-1-AP targets ALDH3A1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit
Immunogen ALDH3A1 fusion protein Ag7977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 3 family, memberA1
Synonyms EC:1.2.1.5, ALDHIII, ALDH3, Aldehyde dehydrogenase, dimeric NADP-preferring, AL3A1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC004370
Gene Symbol ALDH3A1
Gene ID (NCBI) 218
RRIDAB_2273745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30838
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

实验方案

Product Specific Protocols
WB protocol for ALDH3A1 antibody 15578-1-APDownload protocol
IHC protocol for ALDH3A1 antibody 15578-1-APDownload protocol
IF protocol for ALDH3A1 antibody 15578-1-APDownload protocol
IP protocol for ALDH3A1 antibody 15578-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Impact of keratocyte differentiation on corneal opacity resolution and visual function recovery in male rats

Authors - Andri K Riau
View Article
humanWB

Mol Cell

Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.

Authors - Alessandra Fiore
View Article
ratIF

Nat Commun

Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma.

Authors - Bin Kong
View Article
humanIHC

Adv Healthc Mater

Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling.

Authors - Wei Zhang
View Article
humanIF

Biofabrication

Development of a novel bioartificial cornea using 3D bioprinting based on electrospun micro-nanofibrous decellularized extracellular matrix

Authors - Shi-Yao Zhang
View Article
humanWB

Cell Chem Biol

Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.

Authors - Amy Flor
View Article