验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy ALDH3A1 Ready-To-Use IHC Kit. ALDH3A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15578-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-15578 targets ALDH3A1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ALDH3A1 fusion protein Ag7977 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldehyde dehydrogenase 3 family, memberA1 |
| 别名 | Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII |
| 计算分子量 | 50 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC004370 |
| 基因名称 | ALDH3A1 |
| Gene ID (NCBI) | 218 |
| RRID | AB_3083974 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P30838 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ALDH3A1 antibody CL488-15578 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |