• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of HEK-293 using CL594-68036

    FC experiment of HEK-293 using CL594-68036

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ALDH3A1 (CL594-68036, Clone:1D4G7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HEK-293 using CL594-68036

    IF Staining of HEK-293 using CL594-68036

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 ALDH3A1 antibody (CL594-68036, Clone: 1D4G7 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated ALDH3A1 Monoclonal antibody

ALDH3A1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-68036

产品说明书

CloneNo. 1D4G7

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ALDH3A1 Ready-To-Use IHC Kit. ALDH3A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68036-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-68036 targets ALDH3A1 in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 ALDH3A1 fusion protein Ag8125 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldehyde dehydrogenase 3 family, memberA1
别名 Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII
计算分子量 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC004370
基因名称 ALDH3A1
Gene ID (NCBI) 218
RRIDAB_2934794
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP30838
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

实验方案

Product Specific Protocols
IF protocol for CL594 ALDH3A1 antibody CL594-68036Download protocol
FC protocol for CL594 ALDH3A1 antibody CL594-68036Download protocol
Standard Protocols
Click here to view our Standard Protocols