• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80837-1-RR

    WB analysis using 80837-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 80837-1-RR

    WB analysis of HEK-293 using 80837-1-RR

    WB result of ALDOA antibody (80837-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDOA transfected HEK-293 cells.

  • IF Staining of HeLa using 80837-1-RR

    IF Staining of HeLa using 80837-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 80837-1-RR

    IF Staining of HeLa using 80837-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 80837-1-RR

    Affinity and Kinetic Characterization of 80837-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80837-1-RR against Human ALDOA were performed. The affinity constant is 1.34 nM.

  • Featured Product
  • KD/KO验证

ALDOA Recombinant antibody

ALDOA Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 80837-1-RR

产品说明书

CloneNo. 2F4

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse, Pig, rat

应用

WB, IF/ICC, ELISA

别名

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells, HSC-T6 cells, pig liver tissue, pig skeletal muscle tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80837-1-RR targets ALDOA in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse, Pig, rat
免疫原 ALDOA fusion protein Ag1726 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldolase A, fructose-bisphosphate
别名 Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA
计算分子量 39 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC016800
基因名称 ALDOA
Gene ID (NCBI) 226
RRIDAB_2918914
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04075
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

实验方案

Product Specific Protocols
WB protocol for ALDOA antibody 80837-1-RRDownload protocol
IF protocol for ALDOA antibody 80837-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols