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ALDOA Recombinant antibody, PBS Only

ALDOA Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

2F4

Cat No : 80837-1-PBS

Print datasheet

Synonyms

ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase

验证数据展示

  • WB analysis using 80837-1-RR (same clone as 80837-1-PBS)

    WB analysis using 80837-1-RR (same clone as 80837-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80837-1-RR (ALDOA antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80837-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80837-1-RR (same clone as 80837-1-PBS)

    IF Staining of HeLa using 80837-1-RR (same clone as 80837-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 80837-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80837-1-RR (same clone as 80837-1-PBS)

    IF Staining of HeLa using 80837-1-RR (same clone as 80837-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ALDOA antibody (80837-1-RR, Clone: 2F4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80837-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.

产品信息

80837-1-PBS targets ALDOA in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, Mouse, Pig, rat
Immunogen ALDOA fusion protein Ag1726 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aldolase A, fructose-bisphosphate
Synonyms ALDA, ALDOA, Lung cancer antigen NY LU 1, Muscle type aldolase
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC016800
Gene Symbol ALDOA
Gene ID (NCBI) 226
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).