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ALDOB Monoclonal antibody, PBS Only

ALDOB Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2E4

Cat No : 68396-1-PBS

Print datasheet

Synonyms

ALDB, ALDOB, Liver type aldolase

验证数据展示

  • WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

  • WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    WB analysis using 68396-1-Ig (same clone as 68396-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68396-1-Ig (ALDOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 68396-1-Ig (same clone as 68396-1-PBS)

    IF Staining of HepG2 using 68396-1-Ig (same clone as 68396-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALDOB antibody (68396-1-Ig, Clone: 3D2E4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68396-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ALDOB Ready-To-Use IHC Kit. ALDOB primary antibody included.

产品信息

68396-1-PBS targets ALDOB in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, IF, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen ALDOB fusion protein Ag12823 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name aldolase B, fructose-bisphosphate
Synonyms ALDB, ALDOB, Liver type aldolase
Calculated Molecular Weight 316 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC029399
Gene Symbol ALDOB
Gene ID (NCBI) 229
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05062
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A (ALDOA), B (ALDOB), and C (ALDOC). Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183). This antibody may detect ALDOA.